bone marrow cell differentiation  (MedChemExpress)

Journal: Stem Cell Research & Therapy

Article Title: Ionizing radiation-mediated dendritic cell maturation exacerbates inflammatory response of bone marrow mesenchymal stem cells and impairs osteogenesis in radiation-induced jaw injury

doi: 10.1186/s13287-025-04508-x

Figure Lengend Snippet: Schematic conceptualization of this study. IR activated the NF-κB signaling pathway to promote activation and maturation of DCs, amplifying inflammatory signals through the release of pro-inflammatory cytokines. The mDCs triggered oxidative stress of BMSCs, showing increased intracellular ROS levels, inhibited osteogenic differentiation, and enhanced adipogenic differentiation, which accelerated the progression of radiation-induced jaw injury. VitD3-induced tolDCs exhibited IR resistance and anti-inflammatory properties, possessing the therapeutic potential to accelerate bone regeneration in irradiated jaw defects through local administration. (IR: ionizing radiation; mDC: mature dendritic cell; tolDC: tolerogenic dendritic cell; BMSC: bone marrow mesenchymal stem cell; ROS: reactive oxygen species; vitamin D3: vitD3)

Article Snippet: BMSCs were co-cultured with DC-CM and OriCell SD rat bone marrow mesenchymal stem cell adipogenic differentiation basal medium (Cyagen, USA) for 7 days.

Techniques: Activation Assay, Irradiation

Journal: Stem Cell Research & Therapy

Article Title: Ionizing radiation-mediated dendritic cell maturation exacerbates inflammatory response of bone marrow mesenchymal stem cells and impairs osteogenesis in radiation-induced jaw injury

doi: 10.1186/s13287-025-04508-x

Figure Lengend Snippet: Schematic conceptualization of this study. IR activated the NF-κB signaling pathway to promote activation and maturation of DCs, amplifying inflammatory signals through the release of pro-inflammatory cytokines. The mDCs triggered oxidative stress of BMSCs, showing increased intracellular ROS levels, inhibited osteogenic differentiation, and enhanced adipogenic differentiation, which accelerated the progression of radiation-induced jaw injury. VitD3-induced tolDCs exhibited IR resistance and anti-inflammatory properties, possessing the therapeutic potential to accelerate bone regeneration in irradiated jaw defects through local administration. (IR: ionizing radiation; mDC: mature dendritic cell; tolDC: tolerogenic dendritic cell; BMSC: bone marrow mesenchymal stem cell; ROS: reactive oxygen species; vitamin D3: vitD3)

Article Snippet: BMSCs were co-cultured with mDC-CM and OriCell SD rat bone marrow mesenchymal stem cell osteogenic differentiation basal medium (Cyagen, USA).

Techniques: Activation Assay, Irradiation

Journal: Frontiers in Genetics

Article Title: IGF-1 secreted by mesenchymal stem cells affects the function of lymphatic endothelial progenitor cells: a potential strategy for the treatment of lymphedema

doi: 10.3389/fgene.2025.1584095

Figure Lengend Snippet: Characterisation of mesenchymal stem cells (MSCs). We isolated and cultured MSCs from mouse bone marrow without any treatment and studied their relevant characteristics. (A) Representative photographs of MSCs extracted at different time points during culture at 24 h (a) , 48 h (b) and after passaging (c) ; (B,C) Flow cytometric detection of positive markers CD29, CD44 and Sca-1 and negative markers CD45 and CD11b; Oil red O staining (D) , alizarin red staining (E) and alcian blue staining (F) were used to verify the lipogenic, osteogenic and chondrogenic differentiation ability of MSCs, respectively. scale bar 50 µm in (A,D,E) ; scale bar 100 µm in (F) . MSCs, mesenchymal stem or stromal cells.

Article Snippet: When the fusion of the cells reached 90%–100%, the cells were cultured for 2–3 weeks using mouse bone marrow mesenchymal stem cell lipogenic induced differentiation medium (Procell, China) or osteogenic induced differentiation complete medium (Procell, China) according to the protocols.

Techniques: Isolation, Cell Culture, Passaging, Staining

Journal: Frontiers in Genetics

Article Title: IGF-1 secreted by mesenchymal stem cells affects the function of lymphatic endothelial progenitor cells: a potential strategy for the treatment of lymphedema

doi: 10.3389/fgene.2025.1584095

Figure Lengend Snippet: Insulin-like growth factor 1 (IGF-1) secreted by MSC promotes LEPC proliferation and inhibits apoptosis. (A) LEPCs were cultured alone or co-cultured with MSCs for 0 h, 24 h, 48 h or 72 h. Absorbance of LEPCs was measured by CCK-8. **p < 0.01,***p < 0.001 versus LEPCs group; (B,C) LEPCs were co-cultured with MSCs for 72 h. Representative images of EdU + LEPCs and statistical analysis. ***p < 0.001 versus LEPCs group; (D) ELISA for IGF-1 in the culture medium of MSCs or LEPCs. ***p < 0.001 versus MSCs group; (E) Absorbance of LEPCs was detected by CCK-8 after LEPCs were cultured alone, co-cultured with MSCs, or anti-IGF-1 was added to the co-culture system. *p < 0.01 versus LEPCs group; #p < 0.01 versus MSCs + LEPCs group; Cells were then stained with EdU and statistically analysed for positive cells (F,G) . ***p < 0.001 versus LEPCs group; ###p < 0.001 versus MSCs + LEPCs group; (H) CCK-8 experiments to validate the concentration and time dependence of LEPCs on IGF-1. **p < 0.01, ***p < 0.001 versus Control group; (I,J) Immunoblotting of apoptosis-associated proteins and quantification of blotting intensity after 72 h of IGF-1 (100 ng/mL) treatment of LEPCs, GAPDH was used as a loading control. ***p < 0.001 versus LEPCs group; (K) Representative images of apoptotic cells detected by flow cytometry; (L) Quantification of early, late and total apoptotic percentages in untreated or IGF-1-treated LEPCs. *p < 0.05 versus untreated control cells. scale bar 100 µm in B,F; MSCs, mesenchymal stem cells; LEPCs, lymphatic endothelial progenitor cells; IGF-1,insulin-like growth factor 1.

Article Snippet: When the fusion of the cells reached 90%–100%, the cells were cultured for 2–3 weeks using mouse bone marrow mesenchymal stem cell lipogenic induced differentiation medium (Procell, China) or osteogenic induced differentiation complete medium (Procell, China) according to the protocols.

Techniques: Cell Culture, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Staining, Concentration Assay, Control, Western Blot, Flow Cytometry

Journal: Frontiers in Genetics

Article Title: IGF-1 secreted by mesenchymal stem cells affects the function of lymphatic endothelial progenitor cells: a potential strategy for the treatment of lymphedema

doi: 10.3389/fgene.2025.1584095

Figure Lengend Snippet: Combined transplantation of MSCs and LEPCs attenuates hindlimb lymphedema in mice. (A) Images of mouse hind limbs 1 day after surgery; (B) footpad thickness of surgical limbs versus normal limbs in mice,****p < 0.0001; (C) Images of hindlimb oedema in mice at 0, 6 and 12 days after cell transplantation, each set of images from the same mouse; (D) Measurement of footpad thickness in each group of mice after cell transplantation,***p < 0.001 versus other groups; ###p < 0.001 versus LEPCs group; (E) Immunohistochemistry using the lymphatic endothelial marker LYVE-1 in mouse hindlimb samples 12 days after cell transplantation; lymphatic vessels are brown (arrowheads); scale bar 50 µm in a-d,scale bar 20 µm in e-h; (F,G) Analysis of the number of lymphatic vessels and the total area of lymphatic vessels in the immunohistochemical results. *p < 0.05, **p < 0.01 versus PBS group; #p < 0.05 versus LEPCs group. MSCs, mesenchymal stem or stromal cells; LEPCs, lymphatic endothelial progenitor cells; LYVE-1,lymphatic vessel endothelial hyaluronan receptor-1; HPF:high-power field.

Article Snippet: When the fusion of the cells reached 90%–100%, the cells were cultured for 2–3 weeks using mouse bone marrow mesenchymal stem cell lipogenic induced differentiation medium (Procell, China) or osteogenic induced differentiation complete medium (Procell, China) according to the protocols.

Techniques: Transplantation Assay, Immunohistochemistry, Marker, Immunohistochemical staining

Journal: Drug Design, Development and Therapy

Article Title: Extracellular Vesicles Derived from H 2 O 2 -Stimulated Adipose-Derived Stem Cells Alleviate Senescence in Diabetic Bone Marrow Mesenchymal Stem Cells and Restore Their Osteogenic Capacity

doi: 10.2147/DDDT.S454509

Figure Lengend Snippet: Diabetic BMSCs stimulated by H-EVs showed enhanced osteogenesis. ( A ) The expression of ALP in MSCs was visualized using the BCIP/NBT method (scale bar: 100 μm). ( B ) The photographs of ALP staining and ( C ) the quantitative analysis of ALP activity in cell lysates demonstrated consistent results. (n = 6) ( D ) Alizarin Red staining was performed to evaluate calcium deposition in BMSCs (scale bar: 100 μm). ( E ) The ARS in each well was extracted, ( F ) and its absorbance was measured at a wavelength of 570 nm for semi-quantitative analysis (n = 6).

Article Snippet: Adipogenic differentiation of BSMCs was performed using a rat bone marrow mesenchymal stem cells adipogenic differentiation kit according to the manufacturer’s protocol (Cyagen Biosciences Inc., CA, USA).

Techniques: Expressing, Staining, Activity Assay

Journal: Drug Design, Development and Therapy

Article Title: Extracellular Vesicles Derived from H 2 O 2 -Stimulated Adipose-Derived Stem Cells Alleviate Senescence in Diabetic Bone Marrow Mesenchymal Stem Cells and Restore Their Osteogenic Capacity

doi: 10.2147/DDDT.S454509

Figure Lengend Snippet: Preconditioning with H-EVs enhanced the expression of osteogenesis-related genes and proteins in diabetic BMSCs. ( A ) The gene expressions of RUNX2, COL1, OCN and OPN in BMSCs were checked by PCR (n = 5). ( B ) Western blotting was performed to detect the protein expressions of RUNX2, COL1, and OCN in BMSCs. ( C ) Semi-quantitative analysis of the content of RUNX2, COL1, and OCN (n = 5). ( D ) The level of OPN in MSCs was examined using immunofluorescence staining (scale bar: 100 μm).

Article Snippet: Adipogenic differentiation of BSMCs was performed using a rat bone marrow mesenchymal stem cells adipogenic differentiation kit according to the manufacturer’s protocol (Cyagen Biosciences Inc., CA, USA).

Techniques: Expressing, Western Blot, Immunofluorescence, Staining

Journal: Drug Design, Development and Therapy

Article Title: Extracellular Vesicles Derived from H 2 O 2 -Stimulated Adipose-Derived Stem Cells Alleviate Senescence in Diabetic Bone Marrow Mesenchymal Stem Cells and Restore Their Osteogenic Capacity

doi: 10.2147/DDDT.S454509

Figure Lengend Snippet: H-EVs pretreatment alleviate the senescence of diabetic BMSCs. ( A ) Representative images of senescence-associated β-galactosidase-(SA β-gal) staining (Scale bar: 100 μm). ( B ) quantitative analysis of β-gal-positive BMSCs (n = 6). ( C ) Expression of senescence-related genes, including P16, P21 and P53, was detected by PCR (n = 6). ( D ) The level of OPN in MSCs was examined using immunofluorescence staining. ( E ) Quantitative analysis of the proportion of P21-positive cells (n = 5, scale bar: 100 μm).

Article Snippet: Adipogenic differentiation of BSMCs was performed using a rat bone marrow mesenchymal stem cells adipogenic differentiation kit according to the manufacturer’s protocol (Cyagen Biosciences Inc., CA, USA).

Techniques: Staining, Expressing, Immunofluorescence

Journal: Drug Design, Development and Therapy

Article Title: Extracellular Vesicles Derived from H 2 O 2 -Stimulated Adipose-Derived Stem Cells Alleviate Senescence in Diabetic Bone Marrow Mesenchymal Stem Cells and Restore Their Osteogenic Capacity

doi: 10.2147/DDDT.S454509

Figure Lengend Snippet: H-EVs pretreatment alleviated oxidative stress and activated the Nrf2 pathway in diabetic BMSCs. ( A ) The intracellular ROS levels of MSCs was detected using DCFH-DA (scale bar: 100 μm). ( B ) The relative fluorescence intensity of DCFH-DA was quantified using flow analysis; the MDA level and SOD viability of MSCs were determined using commercial kits (n = 6). ( C ) Protein expression of Nrf2 in cell nucleus was detected by Western blotting. ( D ) Semi-quantitative analysis of Nrf2 level (n = 5). ( E ) Protein expression of SOD, NQO1 and HO-1 in the cytosol. ( F ) Semi-quantitative analyses of SOD, NQO1 and HO-1 (n = 6).

Article Snippet: Adipogenic differentiation of BSMCs was performed using a rat bone marrow mesenchymal stem cells adipogenic differentiation kit according to the manufacturer’s protocol (Cyagen Biosciences Inc., CA, USA).

Techniques: Fluorescence, Expressing, Western Blot

Journal: Arthritis Research & Therapy

Article Title: Specific isoforms of the ubiquitin ligase gene WWP2 are targets of osteoarthritis genetic risk via a differentially methylated DNA sequence

doi: 10.1186/s13075-024-03315-8

Figure Lengend Snippet: Investigation of the transcriptional regulatory function of the DMR in Tc28a2 chondrocytes. A Schematic representation of the promoter and enhancer plasmid vector constructs. B Lucia reporter assays assessing promoter or enhancer activity in the presence of construct containing the DMR in an unmethylated or methylated state. Values were normalised to those in empty vector control (dotted horizontal line). Black dots represent individual samples (12 replicates per group). Box plots show the median, 25 th and 75 th percentiles, and minimum and maximum values. P -values calculated by Mann Whitney U test with Holm-Šídák correction. * = P < 0.05; *** = P < 0.001; **** = P < 0.0001

Article Snippet: Human chromatin state data was mined from the ROADMAP Epigenomics Project [ ] to identify regulatory function across the WWP2 /miR-140 locus, focusing on H1 human embryonic stem cell line-derived (H1-derived) mesenchymal stem/stromal cells (MSCs; E006) and chondrocytes differentiated from bone marrow-derived stem/stromal cells (E049).

Techniques: Plasmid Preparation, Construct, Activity Assay, Methylation, Control, MANN-WHITNEY

Journal: Arthritis Research & Therapy

Article Title: Specific isoforms of the ubiquitin ligase gene WWP2 are targets of osteoarthritis genetic risk via a differentially methylated DNA sequence

doi: 10.1186/s13075-024-03315-8

Figure Lengend Snippet: Epigenetic modulation of the DMR in Tc28a2 chondrocytes. A Schematic representation of the 16 CpGs and of the five gRNAs (arrow pointing left, antisense strand; arrow pointing right, sense strand). cg26736200 (CpG8) and cg26661922 (CpG13) are highlighted. B Mean DNAm levels (%) of the 16 CpGs following expression of dCas9-DNMT3a protein in control (black), with non-targeting gRNA, or in samples with a targeting gRNA (coloured). Seven replicates for control and for each targeting gRNA. C Relative expression of the three WWP2 transcripts and of the two miR-140 strands following epigenetic editing with gRNAs. Values were normalised to non-targeting gRNA. P -values were calculated using a paired t -test with Benjamini-Hochberg correction. * = P < 0.05; ** = P < 0.01; ns = not significant ( P > 0.05)

Article Snippet: Human chromatin state data was mined from the ROADMAP Epigenomics Project [ ] to identify regulatory function across the WWP2 /miR-140 locus, focusing on H1 human embryonic stem cell line-derived (H1-derived) mesenchymal stem/stromal cells (MSCs; E006) and chondrocytes differentiated from bone marrow-derived stem/stromal cells (E049).

Techniques: Expressing, Control

Journal: Arthritis Research & Therapy

Article Title: Specific isoforms of the ubiquitin ligase gene WWP2 are targets of osteoarthritis genetic risk via a differentially methylated DNA sequence

doi: 10.1186/s13075-024-03315-8

Figure Lengend Snippet: In-silico analysis of the region harbouring WWP2 , rs34195470 and the DMR. A Schematic representation of chromosome 16q22.1, expanding to show key genomic features proximal to WWP2 including other genes, microRNAs and small nucleolar RNAs (snoRNAs). Three SNPs are marked underneath this: rs6499244, rs34195470 and rs1052429. B The genomic region is further expanded to show the gene structure of the WWP2 isoforms WWP2 -FL, WWP2 -N and WWP2 -C. Horizontal lines represent introns, full height vertical bars represent exons, and half-height vertical bars represent 5’ and 3’ UTRs. The location of miR-140 and the DMR are also shown. Coordinates from UCSC hg19. C Chromatin regulatory state data from ROADMAP for H1-derived embryonic MSCs and chondrocytes. Colours corresponding to the different regulatory elements shown at bottom of figure. D ATAC-sequencing peaks generated from foetal hip, foetal knee, OA hip, and OA knee chondrocytes. Open chromatin regions marked by vertical black lines. E Capture Hi-C chromatin interactions identified at the DMR in H1-derived embryonic MSCs using the 3D Genome Browser, represented as a loop with the flat ends of the loop spanning the width of the interacting regions

Article Snippet: Human chromatin state data was mined from the ROADMAP Epigenomics Project [ ] to identify regulatory function across the WWP2 /miR-140 locus, focusing on H1 human embryonic stem cell line-derived (H1-derived) mesenchymal stem/stromal cells (MSCs; E006) and chondrocytes differentiated from bone marrow-derived stem/stromal cells (E049).

Techniques: In Silico, Derivative Assay, Sequencing, Generated, Hi-C

Journal: Arthritis Research & Therapy

Article Title: Specific isoforms of the ubiquitin ligase gene WWP2 are targets of osteoarthritis genetic risk via a differentially methylated DNA sequence

doi: 10.1186/s13075-024-03315-8

Figure Lengend Snippet: Transcription factors (TFs) predicted to bind at the DMR. A Schematic representation of the 16 CpGs and of 10 TFs predicted to bind at or close to the CpGs. cg26736200 (CpG8) and cg26661922 (CpG13) are highlighted. The TFs are marked by grey rectangles with the direction of the arrows within the rectangles indicating the DNA strand the TF is predicted to bind to (arrows pointing to the left = antisense strand, arrows pointing to the right = sense strand). TF heterodimers are denoted by a double colon (::) between two TFs. B Expression levels (TPM, transcripts per million) of the TFs in cartilage chondrocytes from 10 OA patients. Triangles represent individual samples. Box plots show the median, 25 th and 75 th percentiles, and minimum and maximum values

Article Snippet: Human chromatin state data was mined from the ROADMAP Epigenomics Project [ ] to identify regulatory function across the WWP2 /miR-140 locus, focusing on H1 human embryonic stem cell line-derived (H1-derived) mesenchymal stem/stromal cells (MSCs; E006) and chondrocytes differentiated from bone marrow-derived stem/stromal cells (E049).

Techniques: Expressing